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After cells were incubated at 37 C for 1 h, 0.5 mL of solubilizer was added to the cells, and the resulting solution was mixed gently using an orbital shaker for 1 h at room temperature (20 C)

Its not about following rigid rules, but about understanding the principles that make an outfit work

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The attribution of Thousand Island Dressing's creation may be debated, but both of the supposedly original versions included hard-boiled egg